Diagnostic efficacy of Ziehl-Neelsen method against fluorescent microscopy in detection of acid fast bacilli

Comparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli.

To evaluate Ziehl-Neelsen (ZN) staining using variants of carbol-fuchsin solution, duplicate smears from 416 samples were stained with ZN, one set with 1% basic fuchsin and the other 0.3%. Another set of duplicate smears from 398 samples were stained with ZN, one with 1% basic fuchsin and the other 0.1%. The coded smears were read and discrepancies resolved. All samples underwent mycobacterial ...

Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli.

We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the "gold standard." The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantl...

Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli.

Image Segmentation of Ziehl-Neelsen Sputum Slide Images for Tubercle Bacilli Detection

Tuberculosis (TB) remains one of the leading causes of death in developing countries and its recent resurgences in both developed and developing countries warrants global attention. Globally, there were an estimated of 9.27 million incident cases of TB in 2007. This is an increase from 9.24 million cases in 2006, 8.3 million cases in 2000 and 6.6 million cases in 1990. Most of the estimated num...

A Comparison of Ziehl-Neelsen Staining and Fluorescent Microscopy for Diagnosis of Pulmonary Tuberculosis

According to WHO one third of the world population have tuberculosis. The present study was undertaken to compare the efficacy of flurochrome(FI) stain with conventional Z-N stain in the diagnosis of pulmonary & extrapulmonary tuberculosis. 388 cases of suspected pulmonary tuberculosis were included in the study. All samples were screen for Acid Fast Bacilli (AFB) by Z-N & Fluorescent staining ...